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JoVE Journal Neuroscience
En invasiv metod för aktivering av mus Dentate Gyrus av högfrekvent stimulering
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En invasiv metod för aktivering av mus Dentate Gyrus av högfrekvent stimulering

Article DOI: 10.3791/57857-v • 12:26 min • June 2nd, 2018
June 2nd, 2018 •
1, 1,2
1Department of Neurobiology, Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University

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Detta protokoll visar hur du ställer in en pålitlig HFS metod hos möss. Nervceller i hippocampus dentate gyrus stimuleras elektriskt av HFS direkt och indirekt i vivo. Neuronal aktivitet och molekylär signalering granskas av c-fos och Notch1 Immunofluorescerande färgning, respektive; neurogenes kvantifieras i Bromdeoxiuridin märkning assay.

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Neurovetenskap fråga 136 hög frekvens stimulering dentate gyrus neuronal aktivitet neurogenes BrdU märkning
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