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En invasiv metode for aktivering af musen Dentate Gyrus af højfrekvente Stimulation
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En invasiv metode for aktivering af musen Dentate Gyrus af højfrekvente Stimulation

Article DOI: 10.3791/57857-v • 12:26 min • June 2nd, 2018
June 2nd, 2018 •
1, 1,2
1Department of Neurobiology, Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University

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Denne protokol viser, hvordan du konfigurerer en pålidelig HFS metode i mus. Neuroner i hele den hippocampus dentate gyrus stimuleres elektrisk af HFS direkte og indirekte in vivo. Neuronal aktivitet og molekylær signalering er undersøgt af c-fos og Notch1 immunfluorescent farvning, henholdsvis; neurogenese er kvantificeret ved bromodeoxyuridine mærkning assay.

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Neurovidenskab sag 136 høj frekvens stimulation dentate gyrus neuronal aktivitet neurogenese BrdU mærkning
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