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Neuroscience

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En invasiv metode for aktivering af musen Dentate Gyrus af højfrekvente Stimulation
 

En invasiv metode for aktivering af musen Dentate Gyrus af højfrekvente Stimulation

Article doi: 10.3791/57857
June 2nd, 2018 Usage Statistics

Summary June 2nd, 2018

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Denne protokol viser, hvordan du konfigurerer en pålidelig HFS metode i mus. Neuroner i hele den hippocampus dentate gyrus stimuleres elektrisk af HFS direkte og indirekte in vivo. Neuronal aktivitet og molekylær signalering er undersøgt af c-fos og Notch1 immunfluorescent farvning, henholdsvis; neurogenese er kvantificeret ved bromodeoxyuridine mærkning assay.

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