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JoVE Journal Biochemistry
Korrekturlesing og DNA reparasjon analysen Single nukleotid forlengelse og MALDI-TOF massespektrometri analyse
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Korrekturlesing og DNA reparasjon analysen Single nukleotid forlengelse og MALDI-TOF massespektrometri analyse

Article DOI: 10.3791/57862-v • 11:08 min • June 19th, 2018
June 19th, 2018 •
*1,2, *3, 1, 1, 1, 2, 1,2, 1,2, 1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University
* These authors contributed equally

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En ikke-merket, ikke-radio-isotopanrikning metode for DNA polymerase korrekturlesing og en DNA reparasjon analysen ble utviklet ved hjelp av høy oppløsning MALDI-TOF massespektrometri og en single nukleotid forlengelsen strategi. Analysen viste seg å være svært spesifikke, enkel, rask og lett å utføre for korrekturlesing og reparere patcher kortere enn 9-nukleotider.

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Biokjemi problemet 136 DNA polymerase korrekturlesing DNA-reparasjon MALDI-TOF massespektrometri single nukleotid forlengelsen dideoxyribonucleotide trifosfat DNA mismatch DNA replikasjonsfeil
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