Long-term <em>In Vivo</em> Tracking of Inflammatory Cell Dynamics Within <em>Drosophila</em> Pupae

Helen Weavers; Anna Franz; Will Wood; Paul Martin

doi:10.3791/57871

Journal

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Immunology and Infection

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Langsigtet In Vivo sporing af inflammatoriske celler dynamikken i Drosophila pupper

Long-term <em>In Vivo</em> Tracking of Inflammatory Cell Dynamics Within <em>Drosophila</em> Pupae

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

Langsigtet In Vivo sporing af inflammatoriske celler dynamikken i Drosophila pupper

Please note that all translations are automatically generated. Click here for the English version.

8,720 Views

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09:26 min

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June 14, 2018

DOI:

10.3791/57871-v

DOI:

10.3791/57871-v

•

09:26 min

June 14, 2018

•

4 Views

Helen Weavers^1,2, Anna Franz¹, Will Wood³, Paul Martin^1,4

¹School of Biochemistry, Biomedical Sciences,University of Bristol, ²School of Cellular and Molecular Medicine, Biomedical Sciences,University of Bristol, ³MRC Centre for Inflammation Research,University of Edinburgh, Queens Medical Research Institute, ⁴School of Physiology, Pharmacology, and Neuroscience, Biomedical Sciences,University of Bristol

Transcript

Summary

Her præsenterer vi en protokol til live imaging sår reparation og det tilknyttede inflammatoriske respons på høj spatio-temporal opløsning i vivo. Denne metode udnytter Drosophila puppe udviklingstrin at muliggøre langsigtede imaging og sporing af specifikke cellepopulationer over tid og er kompatibel med effektiv RNAi-medieret genet inaktivering.