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Immunology and Infection

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JoVE Journal Immunology and Infection
Langsigtet In Vivo sporing af inflammatoriske celler dynamikken i Drosophila pupper
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Langsigtet In Vivo sporing af inflammatoriske celler dynamikken i Drosophila pupper

Article DOI: 10.3791/57871-v • 09:26 min • June 14th, 2018
June 14th, 2018 •
1,2, 1, 3, 1,4
1School of Biochemistry, Biomedical Sciences, University of Bristol, 2School of Cellular and Molecular Medicine, Biomedical Sciences, University of Bristol, 3MRC Centre for Inflammation Research, University of Edinburgh, Queens Medical Research Institute, 4School of Physiology, Pharmacology, and Neuroscience, Biomedical Sciences, University of Bristol

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Her præsenterer vi en protokol til live imaging sår reparation og det tilknyttede inflammatoriske respons på høj spatio-temporal opløsning i vivo. Denne metode udnytter Drosophila puppe udviklingstrin at muliggøre langsigtede imaging og sporing af specifikke cellepopulationer over tid og er kompatibel med effektiv RNAi-medieret genet inaktivering.

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Immunologi og infektion sag 136 sår healing betændelse medfødt immunitet Drosophila melanogaster Drosophila puppe hemocyte celle migration live imaging laser ablation Konfokal mikroskopi photoconversion
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