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Journal / Biochemistry / Hurtig Isolation af Mitoribosome fra HEK celler
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Biochemistry

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JoVE Journal Biochemistry
Hurtig Isolation af Mitoribosome fra HEK celler
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Hurtig Isolation af Mitoribosome fra HEK celler

Article DOI: 10.3791/57877-v • 09:33 min • October 4th, 2018
October 4th, 2018 •
1, 1, 1, 1
1Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University

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Mitokondrier har specialiseret ribosomer, der afveg fra deres bakteriel og cytoplasmatisk modstykker. Her viser vi, hvordan mitoribosomes kan fås fra deres indfødte rum i HEK celler. Metoden indebærer isolering af mitokondrier fra suspension celler og deraf følgende rensning af mitoribosomes.

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Biokemi spørgsmål 140 mitokondrier oversættelse ribosomet cryo-EM proteinsyntese biokemi
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