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Journal / Biochemistry / Snelle isolatie van de Mitoribosome van HEK cellen
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Snelle isolatie van de Mitoribosome van HEK cellen
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Snelle isolatie van de Mitoribosome van HEK cellen

Article DOI: 10.3791/57877-v • 09:33 min • October 4th, 2018
October 4th, 2018 •
1, 1, 1, 1
1Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University

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Mitochondriën zijn ribosomen die afweek van hun tegenhangers bacteriële en cytoplasmatische gespecialiseerd. Hier laten we zien hoe de mitoribosomes van hun inheemse compartiment in HEK cellen kan worden verkregen. Bij deze methode wordt isolatie van mitochondriën uit schorsing cellen en daaruit voortvloeiende zuivering van mitoribosomes.

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Biochemie kwestie 140 mitochondriën vertaling ribosoom cryo-EM eiwitsynthese biochemie
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