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Journal / Biochemistry / Rask isolering av Mitoribosome fra HEK celler
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Biochemistry

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Rask isolering av Mitoribosome fra HEK celler
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Rask isolering av Mitoribosome fra HEK celler

Article DOI: 10.3791/57877-v • 09:33 min • October 4th, 2018
October 4th, 2018 •
1, 1, 1, 1
1Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University

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Mitokondrier har spesialisert ribosomer som skilt fra sine bakteriell og cytoplasmatiske kolleger. Her viser vi hvordan mitoribosomes kan fås fra deres eget rom i HEK celler. Metoden innebærer isolering av mitokondrier fra suspensjon celler og påfølgende rensing av mitoribosomes.

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Biokjemi problemet 140 mitokondrier oversettelse ribosom cryo-EM proteinsyntese biokjemi
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