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Journal / Biochemistry / In Situ Måling og korrelation af celle tæthed og…
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Biochemistry

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JoVE Journal Biochemistry
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In Situ Måling og korrelation af celle tæthed og lys Emission af en bioluminescerende bakterier

Article DOI: 10.3791/57881-v • 05:52 min • June 28th, 2018
June 28th, 2018 •
1, 1, 1
1Institute of Biochemistry, Graz University of Technology

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En bioluminescerende bakterier regulere lys produktion gennem en række mekanismer, såsom quorum sensing. Denne nye metode tillader i situ -undersøgelse af bioluminescens og sammenhængen af lys emission til celle tæthed. En kunstig en bioluminescerende Escherichia coli system tillader karakterisering af lux operonmodifikation, Lux proteiner og deres samspil.

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Biokemi sag 136 bioluminescens Escherichia coli Gibson kloning vækstkurven luciferase lux operonen marine bakterier quorum sensing plade læser assay
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