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Neuroscience

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JoVE Journal Neuroscience
Eksogene Administration af Microsomes-associerede Alpha-synuclein aggregater til primære neuroner som en kraftfuld celle Model af fibriller dannelse
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Eksogene Administration af Microsomes-associerede Alpha-synuclein aggregater til primære neuroner som en kraftfuld celle Model af fibriller dannelse

Article DOI: 10.3791/57884-v • 09:16 min • June 26th, 2018
June 26th, 2018 •
1,2, 1, 1
1Bio@SNS Laboratory, Scuola Normale Superiore, 2International School for Advanced Studies (SISSA/ISAS)

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Målet med denne protokol er at give en celle-baseret system, der replikerer dannelsen af alfa-synuclein aggregater i vivo. Intracellulære alpha-synuclein indeslutninger er seedet i primære neuroner ved internalisering og formering af eksogene administreres indfødte microsomes-associerede alpha-synuclein aggregater isoleret fra syge alpha-synuclein Transgene mus.

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Neurovidenskab sag 136 Alpha-synuclein aggregater microsomes primære neuroner Parkinsons sygdom celle model.
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