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Påvisning af Protein subcellulært lokalisering af grøn fluorescens Protein Tagging og 4', 6-Diamidino-2-phenylindole farvning i Caenorhabditis elegans
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Påvisning af Protein subcellulært lokalisering af grøn fluorescens Protein Tagging og 4', 6-Diamidino-2-phenylindole farvning i Caenorhabditis elegans

Article DOI: 10.3791/57914-v • 09:36 min • July 30th, 2018
July 30th, 2018 •
1, 1, 1
1Department of Science, Borough of Manhattan Community College/CUNY

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Denne protokol demonstrerer, hvordan man spore en protein nukleare translokation under varmestress ved hjælp af en grøn fluorescens protein (NGL) fusion protein som en markør og 4', 6-diamidino-2-phenylindole (DAPI) farvning. DAPI farvning protokol er hurtig og bevarer normal god landbrugspraksis og protein subcellulært lokalisering signaler.

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Biologi sag 137 varmestress subcellulært lokalisering ændre nukleare lokalisering grønne fluorescens protein DAPI farvning ethanol fiksering acetone fiksation
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