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Opsporen van Eiwit Subcellular Localisatie door Green fluorescentie eiwit Tagging en 4', 6-Diamidino-2-phenylindole vlekken in Caenorhabditis elegans
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Opsporen van Eiwit Subcellular Localisatie door Green fluorescentie eiwit Tagging en 4', 6-Diamidino-2-phenylindole vlekken in Caenorhabditis elegans

Article DOI: 10.3791/57914-v • 09:36 min • July 30th, 2018
July 30th, 2018 •
1, 1, 1
1Department of Science, Borough of Manhattan Community College/CUNY

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Dit protocol laat zien hoe een eiwit nucleaire translocatie onder hittestress bijhouden met behulp van een groene fluorescentie proteïne (GFP) fusieproteïne als merkstof en 4', 6-diamidino-2-phenylindole (DAPI) kleuring. De DAPI kleuring protocol is snel en behoudt de GFP en eiwit subcellular localisatie-signalen.

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Biologie kwestie 137 hittestress subcellular localisatie wijzigen nucleaire localisatie groene fluorescentie eiwit DAPI kleuring fixatie van ethanol aceton fixatie
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