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JoVE Journal Genetics
Vev-spesifikke miRNA Expression profilering i musen hjertet delene bruke In Situ hybridisering
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Vev-spesifikke miRNA Expression profilering i musen hjertet delene bruke In Situ hybridisering

Article DOI: 10.3791/57920-v • 08:22 min • September 15th, 2018
September 15th, 2018 •
1, 2, 3
1Department of Cell and Developmental Biology, University College London, 2Yale Cardiovascular Research Group, Section of Cardiovascular Medicine, Yale School of Medicine, 3Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Yale School of Medicine

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mikro-RNAs (miRNAs) er korte og svært homologe RNA sekvenser, som post-transcriptional regulatorer av messenger RNAs (mRNAs). Gjeldende miRNA gjenkjenningsmetoder varierer i sensitivitet og spesifisitet. Vi beskriver en protokoll som kombinerer i situ hybridisering og immunostai-samtidige deteksjon av miRNA og protein molekyler på musen hjertet vev deler.

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Genetikk problemet 139 mikro-RNA expression profilering LNA sonde i situ hybridisering immunostai- hjerte-hypertrofi
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