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Journal / Developmental Biology / 改良多向分化效力的常规人多能干细胞的化学逆转与幼稚样态…
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Developmental Biology

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JoVE Journal Developmental Biology
改良多向分化效力的常规人多能干细胞的化学逆转与幼稚样态
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改良多向分化效力的常规人多能干细胞的化学逆转与幼稚样态

Article DOI: 10.3791/57921-v • 09:07 min • June 10th, 2018
June 10th, 2018 •
*1, *1, 1, 1
1Department of Oncology, Division of Pediatric Oncology and Institute for Cell Engineering, Johns Hopkins School of Medicine
* These authors contributed equally

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我们提出一项协议, 以有效, 散装, 快速化学逆转传统的谱系诱导人类多潜能干细胞 (hPSC) 成一个 epigenomically 稳定幼稚的植入啮鼠动物样多潜能状态。该方法在常规 hPSC 线的多向分化上降低了谱系引种基因的表达和显著改善。

Tags

发育生物学 136 期 天真干细胞 人多潜能干细胞 分化 tankyrase 抑制 小分子 啮鼠动物
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