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Bioengineering

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JoVE Journal Bioengineering
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En Orbital ryster kultur i pattedyrsceller i O-formede fartøjer til at producere Uniform aggregeringer

Article DOI: 10.3791/57922-v • 05:40 min • January 7th, 2019
January 7th, 2019 •
1,2, 3, 3, 1,4
1Department of Chemical System Engineering, University of Tokyo, 2Department of Biotechnology, Osaka University, 3Biotech Business Unit, Fukoku Co. Ltd, 4Institute of Industrial Science, University of Tokyo

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Her præsenterer vi en protokol for at bruge O-formede fartøjer, specialiserede for suspension kulturer af cellulære aggregater med orbital ryster. De HEK293 celler dyrkes i denne taske danne mere homogene aggregater end dem, der dyrkes i traditionelle kultur fartøjer.

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Bioteknologi spørgsmålet 143 Suspension kultur orbital ryster masseproduktion pattedyr celle kultur taske samlede
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