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Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Article DOI: 10.3791/57932-v • 10:31 min • July 24th, 2018
July 24th, 2018 •
1, 1, 1, 2, 1
1Department of Agronomy and Horticulture and Center for Plant Science Innovation, University of Nebraska-Lincoln, 2USDA-ARS, Wheat, Sorghum and Forage Research Unit, University of Nebraska-Lincoln East Campus

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Excavation of plant roots from the field as well as processing of samples into endosphere, rhizosphere, and soil are described in detail, including DNA extraction and data analysis methods. This paper is designed to enable other laboratories to use these techniques for the study of soil, endosphere, and rhizosphere microbiomes.

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Isolation Analysis Microbial Communities Soil Rhizosphere Roots Perennial Grass Experiments Diversity Sample Type Treatment Plant Genotype Field Experiments Large Sample Numbers Replication Wash Pan Bucket Plant Sample Details Established Workstation Random Selection Lateral Roots Soil Piercing Root Excavation Labeled Bucket Processing Station Soil Clods Zipper Storage
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