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成像 FITC-葡聚糖作为胞吐作用调节的记者
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成像 FITC-葡聚糖作为胞吐作用调节的记者

Article DOI: 10.3791/57936-v • 04:50 min • June 20th, 2018
June 20th, 2018 •
1, 1, 2, 3, 4, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine, Stanford University

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在这里, 我们详细介绍了一种带电细胞成像的调节胞吐作用方法。该方法利用 FITC-葡聚糖, 在溶酶体相关细胞器中积累, 作为记者。这一简单的方法也允许区分不同模式的调节胞吐作用在细胞难以操纵基因。

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生物学 问题 136 FITC 葡聚糖 调节胞吐作用 复合胞吐作用 活细胞成像 溶酶体相关器 吞饮
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