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Imaging FITC-dekstran som en Reporter regulert Exocytosis
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Imaging FITC-dekstran som en Reporter regulert Exocytosis

Article DOI: 10.3791/57936-v • 04:50 min • June 20th, 2018
June 20th, 2018 •
1, 1, 2, 3, 4, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine, Stanford University

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Her detalj vi en metode for levende celle avbilding av regulert exocytosis. Denne metoden bruker FITC-dekstran, som akkumuleres i lysosome-relaterte organeller, som reporter. Denne enkle metoden kan også skille mellom ulike regulert exocytosis i celler som er vanskelige å manipulere genetisk.

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Biologi problemet 136 FITC-dekstran regulert exocytosis sammensatte exocytosis levende celle bildebehandling lysosome-relaterte organeller pinocytosis
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