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Imaging FITC-dextran som Reporter for regulerede exocytose

Article DOI: 10.3791/57936-v • 04:50 min • June 20th, 2018
June 20th, 2018 •
1, 1, 2, 3, 4, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine, Stanford University

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Her detalje vi en metode for levende celle billeddannelse af regulerede exocytose. Denne metode udnytter FITC-dextran, der ophobes i lysosomet-relaterede organeller, som reporter. Denne simple metode giver også mulighed for at skelne mellem forskellige former for regulerede exocytose i celler, der er vanskelige at manipulere genetisk.

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Biologi sag 136 FITC-dextran regulerede exocytose sammensatte exocytose levende celle imaging lysosomet-relaterede organeller pinocytosis
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