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Imaging FITC-dextran som Reporter för reglerade exocytos

Article DOI: 10.3791/57936-v • 04:50 min • June 20th, 2018
June 20th, 2018 •
1, 1, 2, 3, 4, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine, Stanford University

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Här detalj vi en metod för levande cell avbildning av reglerade exocytos. Denna metod använder FITC-dextran, som ackumuleras i lysosomen-relaterade organeller, som reporter. Den här enkla metoden kan också skilja mellan olika lägen av reglerade exocytos i celler som är svåra att manipulera genetiskt.

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Biologi fråga 136 FITC-dextran reglerade exocytos sammansatta exocytos levande cell imaging Lysosomen-relaterade organeller pinocytosis
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