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Visualizzare l'interazione tra la proteina Qdot-labeled e DNA λ site-specifically modificate a livello di singola molecola

Article DOI: 10.3791/57967-v • 08:56 min • July 17th, 2018
July 17th, 2018 •
*1,2, *1, 1,2, 1,2
1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 2Savaid Medical School, University of Chinese Academy of Sciences
* These authors contributed equally

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Qui, presentiamo un protocollo per studiare le interazioni della DNA-proteina mediante microscopia a fluorescenza riflessione interna totale (TIRFM) usando un substrato di DNA λ site-specifically modificate e un Quantum-dot etichettato proteine.

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Biologia problema 137 imaging di singola molecola interazione DNA-proteina sonde fluorescenti Quantum dot microscopia di fluorescenza di riflessione interna totale modifica site-specific di λ del DNA
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