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JoVE Journal Neuroscience
Malattia di Charcot-Marie-Tooth In Vitro di modellazione di trasfezione motoneuroni primari del Mouse
Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits
Journal (Neuroscience)
Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits
Construction of Local Field Potential Microelectrodes for in vivo Recordings from Multiple Brain Structures Simultaneously
Journal (Neuroscience)
Construction of Local Field Potential Microelectrodes for in vivo Recordings from Multiple Brain Structures Simultaneously
 
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Malattia di Charcot-Marie-Tooth In Vitro di modellazione di trasfezione motoneuroni primari del Mouse

Article DOI: 10.3791/57988-v • 07:43 min • January 7th, 2019
January 7th, 2019 •
1,2, 1, 1,3
1Institut NeuroMyoGène, CNRS UMR5310, INSERM U1217, Université Lyon, 2Unité Fonctionnelle de Neurogénétique Moléculaire, Hospices Civils de Lyon, 3Centre de Biotechnologie Cellulaire, Hospices Civils de Lyon

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L'obiettivo di questa tecnica è quello di preparare una cultura altamente arricchita dei motoneuroni primari (MNs) da murino del midollo spinale. Per valutare le conseguenze delle mutazioni che causano malattie MN, descriviamo qui l'isolamento di questi isolati MNs e loro transfezione di magnetofection.

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Neuroscienze problema 143 motoneuroni motoneuroni spinali disordine neuromuscolare malattia di Charcot-Marie-Tooth neurofilamenti magnetofection neurodegenerazione
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