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Doença de Charcot-Marie-Tooth In Vitro de modelagem por Transfecting Mouse motoneurônios primários

Article DOI: 10.3791/57988-v • 07:43 min • January 7th, 2019
January 7th, 2019 •
1,2, 1, 1,3
1Institut NeuroMyoGène, CNRS UMR5310, INSERM U1217, Université Lyon, 2Unité Fonctionnelle de Neurogénétique Moléculaire, Hospices Civils de Lyon, 3Centre de Biotechnologie Cellulaire, Hospices Civils de Lyon

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O objetivo desta técnica é preparar uma cultura altamente enriquecida de motoneurônios primários (MNs) de murino da medula espinhal. Para avaliar as consequências das mutações causadoras de doenças MN, descrevemos aqui o isolamento destes isolados MNs e seu transfeccao por magnetofection.

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Neurociência edição 143 motoneurônios motoneurônios da coluna vertebral desordem neuromuscular doença de Charcot-Marie-Tooth Neurofilamento magnetofection neurodegeneração
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