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Journal / Cancer Research / Phosphopeptide berigelse kombineret med…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Phosphopeptide berigelse kombineret med etiket-fri kvantitativ massespektrometri til at undersøge Phosphoproteome i Prostata Cancer
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Phosphopeptide berigelse kombineret med etiket-fri kvantitativ massespektrometri til at undersøge Phosphoproteome i Prostata Cancer

Article DOI: 10.3791/57996-v • 12:23 min • August 2nd, 2018
August 2nd, 2018 •
*1,2, *3, 4, 5, 1,2,3,6,7
1Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies, Rutgers University, The State University of New Jersey, 2Graduate Program in Quantitative Biomedicine, School of Graduate Studies, Rutgers University, The State University of New Jersey, 3Department of Medicine, Division of Medical Oncology, Rutgers Robert Wood Johnson Medical School, 4Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute, David Geffen School of Medicine, University of California, Los Angeles, 5Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 6Pharmacology, Rutgers Robert Wood Johnson Medical School, 7Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey
* These authors contributed equally

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Denne protokol beskriver en procedure for at udtrække og berige phosphopeptides fra prostata cancer cellelinjer og væv til en analyse af phosphoproteome via massespektrometri-baseret proteomics.

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Kræftforskning spørgsmålet 138 prostata cancer massespektrometri proteomik fosfoproteomanalyse fosforylering kinaser celle signalering
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