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Journal / Cancer Research / Enriquecimiento de fosfopéptidos acoplada con…
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Cancer Research

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JoVE Journal Cancer Research
Enriquecimiento de fosfopéptidos acoplada con espectrometría de masa libre de etiqueta cuantitativa para investigar la Phosphoproteome en cáncer de próstata
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Enriquecimiento de fosfopéptidos acoplada con espectrometría de masa libre de etiqueta cuantitativa para investigar la Phosphoproteome en cáncer de próstata

Article DOI: 10.3791/57996-v • 12:23 min • August 2nd, 2018
August 2nd, 2018 •
*1,2, *3, 4, 5, 1,2,3,6,7
1Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies, Rutgers University, The State University of New Jersey, 2Graduate Program in Quantitative Biomedicine, School of Graduate Studies, Rutgers University, The State University of New Jersey, 3Department of Medicine, Division of Medical Oncology, Rutgers Robert Wood Johnson Medical School, 4Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute, David Geffen School of Medicine, University of California, Los Angeles, 5Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 6Pharmacology, Rutgers Robert Wood Johnson Medical School, 7Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey
* These authors contributed equally

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Este protocolo describe un procedimiento para extraer y enriquecer fosfotoproteida de líneas celulares de cáncer de próstata o tejidos para un análisis de la phosphoproteome través de proteómica masa basado en la espectrometría.

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Investigación de cáncer número 138 cáncer de próstata espectrometría de masas proteómica fosfoproteómico fosforilación quinasas señalización de las células
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