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Journal / Developmental Biology / Análisis transcriptómico de unicelular de células …
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JoVE Journal Developmental Biology
Análisis transcriptómico de unicelular de células endocrinas pancreáticas de ratón
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Análisis transcriptómico de unicelular de células endocrinas pancreáticas de ratón

Article DOI: 10.3791/58000-v • 10:05 min • September 30th, 2018
September 30th, 2018 •
*1,2, *1,2, *1, *1,3, *1,3, 1
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, 2Academy for Advanced Interdisciplinary Studies, Peking University, 3PKU-Tsinghua-NIBS Graduate Program, Peking University
* These authors contributed equally

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Se describe un método para el aislamiento de las células endocrinas del páncreas embrionarios, neonatales y postnatales seguidos por la secuencia de RNA unicelular. Este método permite el análisis del desarrollo del linaje endocrino pancreático, de células dinámicas heterogeneidad y transcriptómicos.

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Biología del desarrollo número 139 páncreas endocrino células la célula β unicelular RNA-secuenciación expresión génica heterogeneidad celular
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