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Journal / Developmental Biology / Einzellige Transkriptomischen Analysen der Maus…
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Developmental Biology

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JoVE Journal Developmental Biology
Einzellige Transkriptomischen Analysen der Maus endokrinen Zellen der Bauchspeicheldrüse
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Einzellige Transkriptomischen Analysen der Maus endokrinen Zellen der Bauchspeicheldrüse

Article DOI: 10.3791/58000-v • 10:05 min • September 30th, 2018
September 30th, 2018 •
*1,2, *1,2, *1, *1,3, *1,3, 1
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, 2Academy for Advanced Interdisciplinary Studies, Peking University, 3PKU-Tsinghua-NIBS Graduate Program, Peking University
* These authors contributed equally

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Wir beschreiben eine Methode zur Isolierung von endokrinen Zellen aus embryonalen, Neugeborenen- und postnatale Bauchspeicheldrüsen gefolgt von einzelligen RNA Sequenzierung. Diese Methode ermöglicht Analysen der pankreatischen endokrinen Linie Entwicklung, Zell-Heterogenität und transkriptomischen Dynamik.

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Entwicklungsbiologie Ausgabe 139 Bauchspeicheldrüse endokrinen Zellen β-Zelle einzellige RNA-Sequenzierung Genexpression zelluläre Heterogenität
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