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Journal / Biochemistry / Borstemmaskin immobilisert metall affinitet Ture, …
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Biochemistry

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JoVE Journal Biochemistry
Borstemmaskin immobilisert metall affinitet Ture, rekonstituering og analysen av en Polyhistidine merket Metalloenzyme for Undergraduate laboratoriet
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Borstemmaskin immobilisert metall affinitet Ture, rekonstituering og analysen av en Polyhistidine merket Metalloenzyme for Undergraduate laboratoriet

Article DOI: 10.3791/58012-v • 08:02 min • August 23rd, 2018
August 23rd, 2018 •
1, 1
1Department of Chemistry, Muhlenberg College

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Her presenterer vi en protokoll for Borstemmaskin immobilisert metall affinitet kromatografi rensing og påfølgende rekonstituering av en polyhistidine merket, ikke-heme jern bindende dioxygenase egnet for undervisning undervisning laboratoriet.

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Biokjemi problemet 138 Polyhistidine metalloenzyme dioxygenase ikke-heme jern rensing undervisning
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