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Studere Ribonucleotide vedtægter
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Studere Ribonucleotide vedtægter: Strand-specifik påvisning af Ribonucleotides i gær genom og måling Ribonucleotide-induceret mutagenese

Article DOI: 10.3791/58020-v • 09:04 min • July 26th, 2018
July 26th, 2018 •
*1, *1, 1
1Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, NIH, DHHS
* These authors contributed equally

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Ribonucleotides er blandt de mest rigelige ikke-kanoniske nukleotider indarbejdet i genomet i eukaryote nukleare DNA-replikation. Hvis ikke ordentligt fjernes, kan ribonucleotides forårsage DNA-skader og mutagenese. Vi præsenterer her, to eksperimentelle metoder, der bruges til at vurdere overfloden af ribonucleotide indarbejdelse i DNA og dets mutagene virkninger.

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Biologi sag 137 Ribonucleotide vedtægter DNA-replikation RNase H2 alkalisk gel elektroforese udsving test mutagenese
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