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Journal / Genetics / 3dpf 斑马鱼幼虫的高通量 DNA…
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Genetics

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3dpf 斑马鱼幼虫的高通量 DNA 提取及基因分型研究
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3dpf 斑马鱼幼虫的高通量 DNA 提取及基因分型研究

Article DOI: 10.3791/58024-v • 10:12 min • June 29th, 2018
June 29th, 2018 •
1,2, 1, 1,2, 1,2, 1,2, 1, 1, 2, 2, 1, 1,2
1Children's Hospital of Eastern Ontario Research Institute, 2Department of Biology, University of Ottawa

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斑马鱼在生物医学研究中被用作可靠的遗传模型有机体, 特别是随着基因编辑技术的出现。当幼虫表型预计, DNA 提取和基因的鉴定可能是挑战。在这里, 我们描述了一个有效的基因分型程序的斑马鱼幼虫, 通过尾部修剪, 早在72小时后受精。

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遗传学 问题 136 斑马鱼,斑马斑马 基因分型 幼虫鳍修剪 DNA 提取
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