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Errata

Erratum: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

Published: July 1, 2016 doi: 10.3791/5804

Abstract

A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

The changes listed below have been made to Table 1.

1). In the recipe for Solution 2, the Final Concentration of EGTA has been changed from:

EGTA  0.1mM

to:

EGTA  1mM

2). In the recipes for Solution 3 and 4, the Final Concentration of Calcium chloride dihydrate has been changed from:

Calcium chloride dihydrate  0.5µM

to:

Calcium chloride dihydrate  5mM

Protocol

A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

The changes listed below have been made to Table 1.

1). In the recipe for Solution 2, the Final Concentration of EGTA has been changed from:

EGTA  0.1mM

to:

EGTA  1mM

2). In the recipes for Solution 3 and 4, the Final Concentration of Calcium chloride dihydrate has been changed from:

Calcium chloride dihydrate  0.5µM

to:

Calcium chloride dihydrate  5mM

Disclosures

No conflicts of interest declared.

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Cite this Article

Erratum: Isolation of HumanMore

Erratum: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure. J. Vis. Exp. (113), e5804, (2016).

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