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JoVE Journal Neuroscience
En optisk Assay for Synaptic vesikel genanvendelse i kulturperler neuroner overekspression præsynaptiske proteiner
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En optisk Assay for Synaptic vesikel genanvendelse i kulturperler neuroner overekspression præsynaptiske proteiner

Article DOI: 10.3791/58043-v • 09:33 min • June 26th, 2018
June 26th, 2018 •
1, 1, 1, 1
1Institute for Anatomy and Embryology, University Medical Centre Göttingen

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Vi beskriver en optisk assay for synaptic vesikel (SV) genbrug i kulturperler neuroner. Kombinere denne protokol med dobbelt Transfektion udtrykke en præsynaptiske markør og protein af interesse tillader os at lokalisere præsynaptiske websteder, deres synaptic vesikel genanvendelse kapacitet, og bestemme rollen af protein af interesse.

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Neurovidenskab sag 136 synaptiske vesikler genanvendelse synapser neuroner Synaptotagmin-1 hippocampus rotte cellekultur
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