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Journal / Genetics / ホット スポット領域の変異の包括的かつ同時検出のための単一液滴デジタル…
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Genetics

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JoVE Journal Genetics
ホット スポット領域の変異の包括的かつ同時検出のための単一液滴デジタル ポリメラーゼの連鎖反応
Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation
Journal (Genetics)
Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation
Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence
Journal (Genetics)
Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence
 
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ホット スポット領域の変異の包括的かつ同時検出のための単一液滴デジタル ポリメラーゼの連鎖反応

Article DOI: 10.3791/58051-v • 08:23 min • September 25th, 2018
September 25th, 2018 •
1,2, 1, 1, 1,3,4, 1,3,5, 1,6, 1
1Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department, Institut Curie, PSL Research University, 2CNRS UMR144, Institut Curie, PSL Research University, 3Department of Medical Oncology, Institut Curie, PSL Research University, 4University Versailles Saint-Quentin-en-Yvelines, 5University Paris Descartes, 6Inserm U830, Institut Curie, PSL Research University

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ここでは、ドロップオフ ddPCR と加水分解プローブの一意のペアを使用して単一の反作用で対象地域の複数の遺伝子の変異を正確に定量化するためのプロトコルを提案する.

Tags

遺伝学、問題 139、ドロップオフの ddPCR、ホット スポットの突然変異、がん、腫瘍、DNA 診断を循環
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