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JoVE Journal Developmental Biology
Nanopartikel-vermittelten SiRNA Gen-silencing in Erwachsenen Zebrafisch-Herz
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Nanopartikel-vermittelten SiRNA Gen-silencing in Erwachsenen Zebrafisch-Herz

Article DOI: 10.3791/58054-v • 09:12 min • July 29th, 2018
July 29th, 2018 •
1, 2, 1, 1, 2, 1
1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, 2Department of Biomedical Engineering, College of Engineering, Peking University

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Es bleibt eine große Herausforderung, bedingte gen-Knockout oder effektive gen-Knockdown in Erwachsenen Zebrafisch Organe zu entwickeln. Hier berichten wir über ein Protokoll zur Durchführung Nanoparticle vermittelte SiRNA Gen-silencing inmitten der Erwachsenen Zebrafisch, wodurch eine neue Verlustfunktion-Methode zur Untersuchung Erwachsenen Organe im Zebrafisch und sonstige Modellorganismen.

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Entwicklungsbiologie Ausgabe 137 Zebrafisch Erwachsene Herz Regeneration Nanopartikel SiRNA Gen-Silencing- Entwicklungs Biologie Genetik
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