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JoVE Journal Developmental Biology
SiRNA Nanoparticle-mediada-silenciamento no coração de Zebrafish adulto
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SiRNA Nanoparticle-mediada-silenciamento no coração de Zebrafish adulto

Article DOI: 10.3791/58054-v • 09:12 min • July 29th, 2018
July 29th, 2018 •
1, 2, 1, 1, 2, 1
1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, 2Department of Biomedical Engineering, College of Engineering, Peking University

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Continua a ser um grande desafio para desenvolver condicional gene nocaute ou eficaz knockdown do gene-em órgãos adultos do zebrafish. Aqui nós relatamos um protocolo para desempenho siRNA nanoparticle-mediada silenciamento no coração de adultos do zebrafish, proporcionando assim um novo método de perda-de-função para estudar órgãos adultos no zebrafish e outros organismos-modelo.

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Biologia do desenvolvimento questão 137 Zebrafish regeneração de coração adulto nanopartículas siRNA biologia do desenvolvimento silencioso de gene genética
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