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Developmental Biology

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JoVE Journal Developmental Biology
Nanoparticelle-mediata siRNA silenziamento genico nel cuore di Zebrafish adulto
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Nanoparticelle-mediata siRNA silenziamento genico nel cuore di Zebrafish adulto

Article DOI: 10.3791/58054-v • 09:12 min • July 29th, 2018
July 29th, 2018 •
1, 2, 1, 1, 2, 1
1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, 2Department of Biomedical Engineering, College of Engineering, Peking University

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Rimane una grande sfida di sviluppare condizionale gene knock-out o efficace gene-atterramento in organi di zebrafish adulto. Qui segnaliamo un protocollo per la performante siRNA nanoparticella-mediata silenziamento genico nel cuore adulto zebrafish, fornendo così un nuovo metodo di perdita di funzione per lo studio di organi adulti in zebrafish e altri organismi di modello.

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Biologia dello sviluppo problema 137 Zebrafish rigenerazione del cuore adulto nanoparticelle biologia inerente allo sviluppo silenziamento del gene di siRNA genetica
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