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Journal / Developmental Biology / Nanopartikel-medierad siRNA nedtystning i vuxen…
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Developmental Biology

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JoVE Journal Developmental Biology
Nanopartikel-medierad siRNA nedtystning i vuxen zebrafiskar hjärtat
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Nanopartikel-medierad siRNA nedtystning i vuxen zebrafiskar hjärtat

Article DOI: 10.3791/58054-v • 09:12 min • July 29th, 2018
July 29th, 2018 •
1, 2, 1, 1, 2, 1
1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, 2Department of Biomedical Engineering, College of Engineering, Peking University

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Det är fortfarande en stor utmaning att utveckla villkorlig gen-knockout eller effektiva gene-knockdown i vuxen zebrafiskar organ. Här rapporterar vi ett protokoll för presterande nanopartikel-medierad siRNA-nedtystning i vuxen zebrafiskar hjärtat, vilket ger en ny förlust-av-funktion-metod för att studera vuxna organ i zebrafiskar och andra modellorganismer.

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Utvecklingsbiologi fråga 137 zebrafiskar vuxen hjärtat regenerering nanopartiklar siRNA gen tysta utvecklingsmässiga biologi genetik
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