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JoVE Journal Biochemistry
Dynamisk Proteomic og miRNA analyse av Polysomes fra isolert musen hjertet etter Langendorff perfusjon
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Dynamisk Proteomic og miRNA analyse av Polysomes fra isolert musen hjertet etter Langendorff perfusjon

Article DOI: 10.3791/58079-v • 07:54 min • August 29th, 2018
August 29th, 2018 •
1,2,3, 1, 1, 1, 1,2, 1
1The Smidt Heart Institute, Cedars-Sinai Medical Center, 2Advanced Clinical Biosystems Research Institute, Cedars-Sinai Medical Center, 3Institute of Analytical Chemistry of the Czech Academy of Sciences

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Her presenterer vi en protokoll for å utføre polysome profilering på isolerte perfused musen hjertet. Vi beskriver metoder for hjertet perfusjon, polysome profilering og analyse av polysome fraksjoner med hensyn til mRNAs, miRNAs og polysome proteom.

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Biokjemi problemet 138 iskemi og reperfusion dynamisk proteom polysome profilering miRNA mRNA nylig syntetisert proteiner protein oversettelsen sukrose tetthet gradert sedimentering isolerte musen hjertet perfusjon Langendorff perfusjon Proteomikk.
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