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Uttrykk for fluorescerende Fusion proteiner i Murine Ben margtransplantasjon-avledet dendrittiske celler og makrofager
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Uttrykk for fluorescerende Fusion proteiner i Murine Ben margtransplantasjon-avledet dendrittiske celler og makrofager

Article DOI: 10.3791/58081-v • 09:07 min • October 30th, 2018
October 30th, 2018 •
1,2, 1,2,3, 1,2, 1
1Laboratory of Leukocyte Signalling, Institute of Molecular Genetics of the ASCR, 2Faculty of Science, Charles University, 3Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry ASCR

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I denne artikkelen gir vi en detaljert protokoll for uttrykket av fluorescerende fusion proteiner i murine benmarg avledet dendrittiske celler og makrofager. Metoden er basert på signaltransduksjon av benmarg progenitors med retroviral konstruksjoner etterfulgt av differensiering i makrofager og dendrittiske celler i vitro.

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Biologi problemet 140 dendrittiske celler makrofager myeloide celler differensiering murine bein margtransplantasjon celler cytokiner M-CSF GM-CSF virusinfeksjon GFP merket protein
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