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JoVE Journal
Immunology and Infection

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In Vitro Canine Neutrophil Extracellular Trap Formation
 

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

Article DOI: 10.3791/58083
August 24th, 2018

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Summary August 24th, 2018

We describe methods to isolate canine neutrophils from whole blood and visualize NET formation in live neutrophils using fluorescence microscopy. Also described are protocols to quantify NET formation and citrullinated histone H3 (citH3) expression using immunofluorescence microscopy.

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