Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

In Vitro Canine Neutrophil Extracellular Trap Formation

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

Article DOI: 10.3791/58083 09:45 min August 24th, 2018
August 24th, 2018



We describe methods to isolate canine neutrophils from whole blood and visualize NET formation in live neutrophils using fluorescence microscopy. Also described are protocols to quantify NET formation and citrullinated histone H3 (citH3) expression using immunofluorescence microscopy.



In Vitro Canine Neutrophil Extracellular Trap Formation Dynamic Analysis Quantitative Analysis Fluorescence Microscopy NET Formation NET Components Mild Peroxidase Citrullinated Histone H3 Blood Collection Jugular Vein Sodium Heparin Tubes Anticoagulant Mixing Clots Red Cell Aggregates Dulbecco's PBS 3%Dextran Aggregation Sedimentation Erythrocytes Leukocyte-rich Plasma Density Gradient Centrifugation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter