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Journal / Immunology and Infection / In Vitro Formação canina neutrófilos extracelular …
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Immunology and Infection

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JoVE Journal Immunology and Infection
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In Vitro Formação canina neutrófilos extracelular armadilha: Análise dinâmica e quantitativa por microscopia de fluorescência

Article DOI: 10.3791/58083-v • 09:45 min • August 24th, 2018
August 24th, 2018 •
1, 2
1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis

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Podemos descrever métodos para isolar caninos neutrófilos do sangue inteiro e visualizar a formação líquida em neutrófilos ao vivo usando microscopia de fluorescência. Também descritos são protocolos para quantificar a formação líquida e citrulinado histona H3 (citH3) expressão usando microscopia de imunofluorescência.

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Imunologia e infecção edição 138 microscopia de imunofluorescência sepse isolamento de colônias de granulócitos histona citrullination peptidylarginine deiminase
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