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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Formation canine piège extracellulaire neutrophiles 
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In Vitro Formation canine piège extracellulaire neutrophiles : Analyse dynamique et Quantitative par microscopie de Fluorescence

Article DOI: 10.3791/58083-v • 09:45 min • August 24th, 2018
August 24th, 2018 •
1, 2
1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis

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Nous décrivons des méthodes pour isoler canines neutrophiles du sang total et de visualiser la formation nette des neutrophiles vivants à l’aide de la microscopie de fluorescence. Également décrites sont des protocoles pour quantifier la formation nette et citrullinés histone H3 (citH3) expression à l’aide de la microscopie par immunofluorescence.

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Immunologie et Infection numéro 138 la microscopie par Immunofluorescence septicémie isolement de granulocyte histone citrullination peptidylarginine déiminase
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