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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Differensiering av mus granulocytt-macrophage-koloni-stimulerende faktor (GM-CSF)-produserende T Helper (THGM) celler
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In Vitro Differensiering av mus granulocytt-macrophage-koloni-stimulerende faktor (GM-CSF)-produserende T Helper (THGM) celler

Article DOI: 10.3791/58087-v • 10:28 min • September 10th, 2018
September 10th, 2018 •
1,2,3, 3, 1,2
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Immunology Programme, Life Sciences Institute, National University of Singapore, 3Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine, National University of Singapore

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Her presenterer vi en protokoll for å skille murine granulocyte-macrophage-colony-stimulating-factor-producing T hjelper (THGM) celler fra naiv CD4 + T celler, inkludert isolering av naiv CD4 + T celler, differensiering av THGM, og analyse av differensierte THGM celler. Denne metoden kan brukes til studier av regulering og funksjon av THGM celler.

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Immunologi og infeksjon utstede 139 THGM T hjelper celle differensiering GM-CSF naiv T celle neuroinflammation inflammatorisk cytokin
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