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JoVE Journal Immunology and Infection
In Vitro Differentiering av mus granulocyt-makrofag-granulocytkolonistimulerande faktor (GM-CSF)-celler T Helper (THGM)
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In Vitro Differentiering av mus granulocyt-makrofag-granulocytkolonistimulerande faktor (GM-CSF)-celler T Helper (THGM)

Article DOI: 10.3791/58087-v • 10:28 min • September 10th, 2018
September 10th, 2018 •
1,2,3, 3, 1,2
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Immunology Programme, Life Sciences Institute, National University of Singapore, 3Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine, National University of Singapore

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Här presenterar vi ett protokoll för att skilja murina granulocyte-macrophage-colony-stimulating-factor-producing T helper (THGM) celler från naiva CD4 + T-celler, inklusive isolering av naiva CD4 + T-celler, differentiering av THGM, och analys av differentierade THGM celler. Denna metod kan tillämpas på studier av förordningen och funktion av THGM celler.

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Immunologi och infektion utfärda 139 THGM T helper cell differentiering GM-CSF naiva T cell neuroinflammation inflammatorisk cytokin
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