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Empfindliche Messung des Mitophagy von Flow Cytometry Verwendung der pH-abhängigen fluoreszierende Reporter Mt-Otamegane
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Empfindliche Messung des Mitophagy von Flow Cytometry Verwendung der pH-abhängigen fluoreszierende Reporter Mt-Otamegane

Article DOI: 10.3791/58099-v • 09:14 min • August 12th, 2018
August 12th, 2018 •
*1,2, *1,2, 3, 1,2
1Peripheral Neuropathy Research Center, College of Medicine, Dong-A University, 2Department of Biochemistry, College of Medicine, Dong-A University, 3Aging Institute, Department of Medicine, University of Pittsburgh School of Medicine
* These authors contributed equally

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Mitophagy, den selektiven Abbau von Mitochondrien, hat im mitochondrialen Homöostase in Verbindung gebracht und ist in den verschiedenen menschlichen Krankheiten dereguliert. Bequeme experimentelle Methoden zur Messung Mitophagy Aktivität sind jedoch sehr beschränkt. Hier bieten wir eine sensible Test zur Messung der Mitophagy Tätigkeit mit Durchflusszytometrie.

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Biologie Ausgabe 138 Mitophagy Mitochondrien Mt-Otamegane Durchflusszytometrie fluoreszierenden Proteins pH-abhängigen Sonde
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