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Biochemistry

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JoVE Journal Biochemistry
Un test In Vitro per rilevare attività di tRNA-Isopentenyl Transferase
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Journal (Biochemistry)
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
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Journal (Biochemistry)
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Un test In Vitro per rilevare attività di tRNA-Isopentenyl Transferase

Article DOI: 10.3791/58100-v • 07:46 min • October 8th, 2018
October 8th, 2018 •
*1, *1, 2, 3, 1, 1
1Department of Biology, Ball State University, 2Department of Genome Sciences, University of Washington, 3Department of Molecular, Cellular, and Developmental Biology, University of Michigan
* These authors contributed equally

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Qui, descriviamo un protocollo per la caratterizzazione biochimica dell'enzima modificante la RNA lievito, Mod5 e discutere come questo protocollo potrebbe essere applicato ad altri enzimi modificanti la RNA.

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Biochimica problema 140 modifica di RNA tRNA isopentenyl transferasi MOD5 TRIT1 ho6A ho6A37 DMAPP
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