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JoVE Journal Biochemistry
Functionele karakterisering van Carboxylesterases in Insecticide resistente huis vliegt, Musca Domestica
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Functionele karakterisering van Carboxylesterases in Insecticide resistente huis vliegt, Musca Domestica

Article DOI: 10.3791/58106-v • 08:17 min • August 23rd, 2018
August 23rd, 2018 •
1, 1
1Department of Entomology and Plant Pathology, Auburn University

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Hier presenteren we een protocol om te produceren huis vliegen carboxylesterase eiwitten in vitro met een baculovirus gemedieerde insect cel expressie systeem en later functioneel karakteriseren van hun rollen in metaboliseringssysteem permethrin, daarmee overdracht van pyrethroïde weerstand door het uitvoeren van cel-gebaseerde MTT assay en in vitro metabole studies.

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Biochemie kwestie 138 Insecticide resistentie carboxylesterases insect cel baculovirus-gemedieerde expressie systeem bepaling van de cytotoxiciteit in vitro metabolisme assay
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