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Hele cellen elektrofysiologi primære kulturperler Murine Enterochromaffin celler

Article DOI: 10.3791/58112-v • 10:04 min • September 26th, 2018
September 26th, 2018 •
*1, *1, 2, 2, 1, 1
1Enteric Neuroscience Program, Division of Gastroenterology & Hepatology,Department of Physiology & Biomedical Engineering, Mayo Clinic, 2Division of Gastroenterology, Department of Medicine, University of Massachusetts Medical School
* These authors contributed equally

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Enterochromaffin (EC) celler utgjør en undergruppe av gastrointestinale epitelceller. EC celler er elektrisk nervøs og slipp serotonin, men vanskeligheter i dyrking og identifisere EC celler har begrenset fysiologiske studier. Metoden som presenteres her oppretter en primær kultur modell mottakelig for undersøkelse av EC enkeltceller av elektrofysiologi.

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Biologi problemet 139 fordøyelsessystemet epitel enterochromaffin celle primære kultur hele cellen elektrofysiologi spenning klemme gjeldende klemme
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