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Journal / Biochemistry / Plade-baserede storstilet dyrkning af…
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Biochemistry

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JoVE Journal Biochemistry
Plade-baserede storstilet dyrkning af Caenorhabditis elegans
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Plade-baserede storstilet dyrkning af Caenorhabditis elegans: forberedelse af prøver til undersøgelse af stofskifteforandringer i Diabetes

Article DOI: 10.3791/58117-v • 05:08 min • August 24th, 2018
August 24th, 2018 •
1, 2,3, 1, 1,4, *1, *1
15th Medical Department, Medical Faculty Mannheim, Heidelberg University, 2Department of Internal Medicine, Heidelberg University, 3German Center for Diabetes Research (DZD), 4European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University
* These authors contributed equally

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Denne protokol beskriver en metode til storstilet dyrkning af Caenorhabditis elegans på solid media. Som et alternativ til flydende kultur, giver denne protokol mulighed for at opnå parametre af forskellige skalaer under plade-baseret dyrkning. Dette øger sammenligneligheden af resultater ved at udelade de morfologiske og metaboliske forskelle mellem flydende og fast MedieKultur.

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Biokemi spørgsmålet 138 C. elegans omfattende kultur reaktiv metabolitter avanceret glykering slutprodukter reaktive ilt arter protein extract hele kroppen lysate plade kultur vask protokol masse massespektrometri diabetisk komplikationer
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