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Biochemistry

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JoVE Journal Biochemistry
Plate-baserte stor skala dyrking av Caenorhabditis elegans
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Journal (Biochemistry)
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Journal (Biochemistry)
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Plate-baserte stor skala dyrking av Caenorhabditis elegans: Sample forberedelse for studiet av metabolske forandringer i Diabetes

Article DOI: 10.3791/58117-v • 05:08 min • August 24th, 2018
August 24th, 2018 •
1, 2,3, 1, 1,4, *1, *1
15th Medical Department, Medical Faculty Mannheim, Heidelberg University, 2Department of Internal Medicine, Heidelberg University, 3German Center for Diabetes Research (DZD), 4European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University
* These authors contributed equally

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Denne protokollen beskriver en metode for storskala dyrking av Caenorhabditis elegans på solid medier. Som et alternativ til flytende kultur kan denne protokollen få parametere av forskjellige skalaer under plate-baserte dyrking. Dette øker sammenlignbarheten resultater ved å utelate morfologiske og metabolske forskjellene mellom flytende og fast mediekultur.

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Biokjemi problemet 138 C. elegans store kultur reaktive metabolitter avanserte glycation end produkter reaktive oksygen arter protein ekstrakt hele kroppen lysate plate kultur vaske protokollen masse massespektrometri diabetiker komplikasjoner
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