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Journal / Genetics / Endogene Protein Tagging i menneskelige induceret …
JoVE Journal
Genetics
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JoVE Journal Genetics
Endogene Protein Tagging i menneskelige induceret pluripotente stamceller ved hjælp af CRISPR/Cas9
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Endogene Protein Tagging i menneskelige induceret pluripotente stamceller ved hjælp af CRISPR/Cas9

Article DOI: 10.3791/58130-v • 14:49 min • August 25th, 2018
August 25th, 2018 •
1, 1, 1, 1, 1, 1
1Allen Institute for Cell Science

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Beskrevet her er en protokol til tagging endogent udtrykte proteiner med fluorescerende tags i menneskelige inducerede pluripotente stamceller ved hjælp af CRISPR/Cas9. Derfor redigerede celler er beriget af fluorescens aktiveret celle sortering og klonede cellelinjer genereres.

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Genetik spørgsmålet 138 stamceller hiPSC Molekylærbiologisk Institut CRISPR Cas9 genom Engineering Gene Knock-i Gene Tagging fluorescerende proteiner
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